Please use this identifier to cite or link to this item: https://physrep.ff.bg.ac.rs/handle/123456789/568
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dc.contributor.authorConway, Gillian Een
dc.contributor.authorHe, Zhongleien
dc.contributor.authorHutanu, Ana Lacramioaraen
dc.contributor.authorCribaro, George Paulen
dc.contributor.authorManaloto, Elineen
dc.contributor.authorCasey, Alanen
dc.contributor.authorTraynor, Damienen
dc.contributor.authorMilosavljević, Vladimiren
dc.contributor.authorHowe, Orlaen
dc.contributor.authorBarcia, Carlosen
dc.contributor.authorMurray, James Ten
dc.contributor.authorCullen, Patrick Jen
dc.contributor.authorCurtin, James Fen
dc.date.accessioned2022-07-12T16:01:37Z-
dc.date.available2022-07-12T16:01:37Z-
dc.date.issued2019en
dc.identifier.urihttps://physrep.ff.bg.ac.rs/handle/123456789/568-
dc.description.abstractRoom temperature Cold Atmospheric Plasma (CAP) has shown promising efficacy for the treatment of cancer but the exact mechanisms of action remain unclear. Both apoptosis and necrosis have been implicated as the mode of cell death in various cancer cells. We have previously demonstrated a caspase-independent mechanism of cell death in p53-mutated glioblastoma multiforme (GBM) cells exposed to plasma. The purpose of this study was to elucidate the molecular mechanisms involved in caspase-independent cell death induced by plasma treatment. We demonstrate that plasma induces rapid cell death in GBM cells, independent of caspases. Accumulation of vesicles was observed in plasma treated cells that stained positive with acridine orange. Western immunoblotting confirmed that autophagy is not activated following plasma treatment. Acridine orange intensity correlates closely with the lysosomal marker Lyso TrackerTM Deep Red. Further investigation using isosurface visualisation of confocal imaging confirmed that lysosomal accumulation occurs in plasma treated cells. The accumulation of lysosomes was associated with concomitant cell death following plasma treatment. In conclusion, we observed rapid accumulation of acidic vesicles and cell death following CAP treatment in GBM cells. We found no evidence that either apoptosis or autophagy, however, determined that a rapid accumulation of late stage endosomes/lysosomes precedes membrane permeabilisation, mitochondrial membrane depolarisation and caspase independent cell death.en
dc.language.isoenen
dc.relation.ispartofScientific reportsen
dc.subject.meshGlioblastomaen
dc.subject.meshLysosomesen
dc.subject.meshPlasma Gasesen
dc.titleCold Atmospheric Plasma induces accumulation of lysosomes and caspase-independent cell death in U373MG glioblastoma multiforme cellsen
dc.typeJournal Articleen
dc.typeResearch Support, Non-U.S. Gov'ten
dc.identifier.doi10.1038/s41598-019-49013-3en
dc.identifier.pmid31501494en
dc.identifier.scopus2-s2.0-85071991015en
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85071991015en
dc.relation.issue1en
dc.relation.volume9en
dc.relation.firstpage12891en
item.openairetypeJournal Article-
item.openairetypeResearch Support, Non-U.S. Gov't-
item.cerifentitytypePublications-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
crisitem.author.orcid0000-0002-7805-5189-
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